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mastercycler nexus gradient  (Eppendorf AG)


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    Structured Review

    Eppendorf AG mastercycler nexus gradient
    Mastercycler Nexus Gradient, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eppendorf+mastercycler+nexus+gradient/Eppendorf/pmc13066795-103-56-62
    Average 99 stars, based on 201853 article reviews
    mastercycler nexus gradient - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical Klebsiella pneumoniae Isolates from Patients of District Hospital in Central Poland.
    Article Snippet: Simplex PCR for each gene, except blaCTX-M genes, was performed in a 25 μL volume containing 1 μL of DNA template, 12.5 μL of the PCR Mix Plus HGC (0.1 U/μL of Taq DNA polymerase, 4 mM of MgCl2, 0.5 mM of each dATP, dCTP, dGTP and dTTP) (A&A Biotechnology, Gdynia, Poland) and gene-specific primers at a final concentration of 200 nM. .. Thermal cycling conditions were as follows: pre-denaturation at 95 ◦C for 4 min; 35 cycles of denaturation at 95 ◦C for 0.5 min, primer annealing at 58 ◦C for 0.5 min, and extension at 72 ◦C for 1 min. Amplification was carried out in the Eppendorf Mastercycler Nexus Gradient (Hamburg, Germany). ..

    Article Title: Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical Klebsiella pneumoniae Isolates from Patients of District Hospital in Central Poland
    Article Snippet: Simplex PCR for each gene, except bla CTX-M genes, was performed in a 25 μL volume containing 1 μL of DNA template, 12.5 μL of the PCR Mix Plus HGC (0.1 U/μL of Taq DNA polymerase, 4 mM of MgCl 2 , 0.5 mM of each dATP, dCTP, dGTP and dTTP) (A&A Biotechnology, Gdynia, Poland) and gene-specific primers at a final concentration of 200 nM. .. Thermal cycling conditions were as follows: pre-denaturation at 95 °C for 4 min; 35 cycles of denaturation at 95 °C for 0.5 min, primer annealing at 58 °C for 0.5 min, and extension at 72 °C for 1 min. Amplification was carried out in the Eppendorf Mastercycler Nexus Gradient (Hamburg, Germany). ..

    Article Title: Comparative Analysis of DNA Extraction Protocols for Molecular Study of Fall Armyworm
    Article Snippet: .. The amplification condition includes the following: initial denaturation for 3 minutes at 95°C; 40 cycles of denaturation for 15 seconds at 95°C; annealing for 20 seconds at 56 °C and elongation for 25 seconds at 72 °C; followed by a final extension step of 10 minutes at 72°C using Eppendorf Mastercycler nexus gradient. ..

    Polymerase Chain Reaction:

    Article Title: Microbial consortia producing dipicolinic acid and methods for selecting microbes for co-formulation with carriers
    Article Snippet: .. Polymerase chain reaction (PCR) was carried out using the Eppendorf Mastercycler® nexus gradient using the recommended thermal cycling conditions from Bio-Rad's ddPCR Supermix for Probes. ..

    Article Title: Effects of Preharvest Application of Oxalic Acid, γ-Aminobutyric Acid, and Melatonin on the Microbiological and Physicochemical Quality of Dried Figs at Commercial Harvest and During Storage.
    Article Snippet: .. PCR reactions were performed in an Eppendorf Mastercycler® nexus gradient (Madrid, Spain) under the conditions described by Gallardo et al. [61] for Bt, Perrone et al. [58] for CaM, by Woudenberg et al. [62] for gpd, and Galán et al. [9] for ITS. ..

    Article Title:  Desmopuntius mahakamensis , a new cyprinid species (Teleostei, Cyprinidae) from East Kalimantan, Indonesia.
    Article Snippet: .. PCR was carried out on an Eppendorf Mastercycler Nexus Gradient in a 25 μl reaction containing 15.5 μl sterile water, 5 μl 5x MyTaq reaction buffer, 0.5 μl MyTaq DNA poly- 373ZooKeys 1256: 371–391 (2025), DOI: 10.3897/zookeys.1256.158411 merase, 0.5 μl of each primer (10 μM), and 2 μl genomic DNA. ..

    Article Title: DNA barcoding-based assessment of genetic variation in selected Southwest Nigerian medicinal Senna species (Caesalpinoideae: Fabaceae)
    Article Snippet: .. PCR were performed by subjecting the samples to thermal cycling conditions following Eppendorf Mastercycler nexus gradient 230: initial denaturation at 95°C for 5 minutes followed by 35 cycles, denaturation at 95°C for 30 seconds, annealing at 50°C for 1 minute and 68°C for 90 secs with a final 10 mins extension step at 68°C and hold at 4°C. ..

    Sterility:

    Article Title:  Desmopuntius mahakamensis , a new cyprinid species (Teleostei, Cyprinidae) from East Kalimantan, Indonesia.
    Article Snippet: .. PCR was carried out on an Eppendorf Mastercycler Nexus Gradient in a 25 μl reaction containing 15.5 μl sterile water, 5 μl 5x MyTaq reaction buffer, 0.5 μl MyTaq DNA poly- 373ZooKeys 1256: 371–391 (2025), DOI: 10.3897/zookeys.1256.158411 merase, 0.5 μl of each primer (10 μM), and 2 μl genomic DNA. ..



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